Silencing of yellow head virus replication in penaeid shrimp cells by dsRNA.

Therapeutic inhibition of yellow head virus multiplication in infected shrimps by YHV-protease dsRNA.

Yellow head virus (YHV) is an invertebrate nidovirus which causes a severe mortality in cultured Penaeus monodon. The mortality may be prevented by prior treatment of shrimps with YHV-protease dsRNA. Whether the YHV infected shrimp might be cured by the dsRNA remains to be investigated. P. monodon injected with 10(-6) YHV showed a high virus replication and mortality within 2 days. Injection of...

Development of real-time PCR assays for detection of white spot syndrome virus, yellow head virus, Taura syndrome virus, and infectious hypodermal and hematopoietic necrosis virus in penaeid shrimp

The Penaeid Shrimp Viral Pandemics due to IHHNV, WSSV, TSV and YHV: History in the Americas and Current Status

At least four virus caused pandemics have adversely affected the global penaeid shrimp farming industry since 1980. These viruses in the approximate order of their discovery are Infectious hypodermal and hematopoietic necrosis virus (IHHNV), Yellow Head Virus (YHV), Taura Syndrome Virus (TSV), and White Spot Syndrome Virus (WSSV). The socioeconomic impact of the diseases caused by these viruses...

Yellow-head virus: a rhabdovirus-like pathogen of penaeid shrimp

Yellow-head virus (YHV), a highly virulent virus of cultured penaeid shrimp, was originally isolated from the black tiger shrimp Penaeus monodon in Thailand. It was initially described as a baculovirus, but was recently reported to be a n RNA-containing virus. The present study reaffirms the genome of highly purified YHV to be an unsegmented single-stranded RNA with negative polarity and of app...

YHV-protease dsRNA inhibits YHV replication in Penaeus monodon and prevents mortality.

Yellow head virus infects cultured shrimps and causes severe mortality resulting in a great economic loss. Haemolymph injection of dsRNA(pro) corresponding to the protease motif of YHV genome resulted in a complete inhibition of YHV replication. The effect of dsRNA lasted for at least 5 days. Injecting sequence-unrelated dsRNA(gfp) or dsRNA(TSV-pol) also resulted in an inhibition of YHV replica...